TY  - CHAP
U1  - Konferenzveröffentlichung
A1  - Erichson, Felix
A1  - Steffen, Fabio D.
A1  - Börner, Richard
T1  - FRET-assisted structural model of the GAAA RNA tetraloop receptor
T2  - 26. Interdisziplinäre Wissenschaftliche Konferenz Mittweida
N2  - Long-range tertiary interactions between RNA tetraloops and their receptors stabilize the folding of ribosomal RNA and support the maturation of the ribosome. Here, we use FRET-assisted structure prediction to develop a structural model of the GAAA tetraloop receptor (TLR) interaction and its dynamics. We build the docked TLR de novo, label the RNA in silico and compute FRET histograms based on MD simulations. The predicted mean FRET efficiency is remarkably consistent with single-molecule experiments of the docked tetraloop. This hybrid approach of experiment and simulation will promote the elucidation of dynamic RNA tertiary contacts and accelerate the discovery of novel RNA and RNA-protein interactions as potential future drug targets.
KW  - Fluoreszenz-Resonanz-Energie-Transfer
KW  - RNS
KW  - MD simulation
KW  - hybrid modeling
KW  - GAAA tetraloop
Y1  - 2021
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:mit1-opus4-122837
SN  - 1437-7624
SS  - 1437-7624
U6  - https://doi.org/10.48446/opus-12283
DO  - https://doi.org/10.48446/opus-12283
IS  - 002
SP  - 230
EP  - 233
S1  - 4
PB  - Hochschule Hochschule Mittweida
CY  - Mittweida
ER  -