@inproceedings{ErichsonSteffenBoerner2021,
  author    = {Felix Erichson and Fabio D. Steffen and Richard B{\"o}rner},
  title     = {FRET-assisted structural model of the GAAA RNA tetraloop receptor},
  series = {26. Interdisziplin{\"a}re Wissenschaftliche Konferenz Mittweida},
  number    = {002},
  publisher = {Hochschule Hochschule Mittweida},
  address   = {Mittweida},
  issn      = {1437-7624},
  doi       = {10.48446/opus-12283},
  url       = {https://nbn-resolving.org/urn:nbn:de:bsz:mit1-opus4-122837},
  pages     = {230 -- 233},
  year      = {2021},
  abstract  = {Long-range tertiary interactions between RNA tetraloops and their receptors stabilize the folding of ribosomal RNA and support the maturation of the ribosome. Here, we use FRET-assisted structure prediction to develop a structural model of the GAAA tetraloop receptor (TLR) interaction and its dynamics. We build the docked TLR de novo, label the RNA in silico and compute FRET histograms based on MD simulations. The predicted mean FRET efficiency is remarkably consistent with single-molecule experiments of the docked tetraloop. This hybrid approach of experiment and simulation will promote the elucidation of dynamic RNA tertiary contacts and accelerate the discovery of novel RNA and RNA-protein interactions as potential future drug targets.},
  language  = {en}
}