TY - CHAP U1 - Konferenzveröffentlichung A1 - Michaelsen, Hannes A1 - Börner, Richard T1 - Optimization of an optical setup for combined TIRF/confocal fluorescence microscopy T2 - 26. Interdisziplinäre Wissenschaftliche Konferenz Mittweida N2 - This work deals with the construction of a microscope for combined total internal reflection fluorescence (TIRF) and confocal microscopy. It is especially designed for single-molecule fluorescence spectroscopy. The design of the microscope body is based on the miCube (Hohlbein lab, Wageningen University, NL). The excitation and detection pathways were adapted to allow both TIRF and confocal illumination as well as camera and pointdetection for two color-channels to allow single-molecule Förster resonance transfer measurements KW - Mikroskopie KW - Konfokale Mikroskopie KW - TIRFM Y1 - 2021 SN - 1437-7624 SS - 1437-7624 U6 - https://doi.org/10.48446/opus-12286 DO - https://doi.org/10.48446/opus-12286 IS - 002 SP - 234 EP - 236 S1 - 3 PB - Hochschule Mittweida CY - Mittweida ER -