@inproceedings{PrudnikowLeidenfrostWestphaletal.2021, author = {Lisa Prudnikow and Robert Leidenfrost and Catrin Westphal and R{\"o}bbe W{\"u}nschiers}, title = {Development of a genetic biomonitoring test for the investigation of pollinator-plant-interactions}, series = {26. Interdisziplin{\"a}re Wissenschaftliche Konferenz Mittweida}, number = {002}, publisher = {Hochschule Mittweida}, address = {Mittweida}, issn = {1437-7624}, doi = {10.48446/opus-12289}, url = {https://nbn-resolving.org/urn:nbn:de:bsz:mit1-opus4-122897}, pages = {244 -- 247}, year = {2021}, abstract = {There is a world-wide decline in biodiversity recorded. Especially insects and accompanying pollinators are threatened. When the foraging behaviour of pollinators is understood in detail, future crop and floral pollination services can be sustained and it is possible to establish projects for the conservation of pollinators and plant biodiversity. With the use of nanopore sequencing methods it is possible to detect pollen species that were collected by pollinators by their genetic information. In this study, a protocol for portable nanopore sequencing of DNA from pollen that was collected by honey bees, bumble bees and wild bees is being designed. DNAmetabarcoding is used to identify species within the mixed DNA sample. The ITS2-region will be used as a barcode. We will investigate pollen preferences of three pollinator species by placing their hives or nests at the same. Based on the results, landscape management schemes are developed that target pollen preferences and nutritional requirements of managed and wild social bee species as well as solitary wild bees.}, language = {en} }