@phdthesis{Leidenfrost2015,
  type      = {Master Thesis},
  author    = {Robert Leidenfrost},
  title     = {Cell-free protein synthesis : establishment of a synthetic biology tool},
  year      = {2015},
  abstract  = {Cell-free protein synthesis is gaining increasing importance in basic and applied research as a result of advances that have been made in the past years. This study successfully established a commercially available coupled in vitro transcription/translation system for DNA expression templates based on HeLa cell lysate and suitable detection methods. Possibilities to establish an inexpensive in-house CFPS system based on E. coli lysate were elucidated. Cell-free extract from E. coli strains HB101, JM109 and B (Luria) was generated using sonication and a streamlined procedure for the creation of a reaction mix for convenient and simple application in CFPS systems was established. Of the three strains tested using the in-house protocol, neither exhibited any CFPS activity.},
  language  = {de}
}