TY - THES U1 - Master Thesis A1 - Beier, Maria T1 - Structure development of the heat shock protein 27 by analyzing the evolutionary and functional relationships to other heat shock proteins N2 - After the expression of the titin-Hsp27-construct with the following purification supplies no satisfied results which makes the realization of the atomic force microscopy not possible. The devel-opment of the structure model by using different bioinformatic methods can establish a model for the protein sequence. As bioinformatic methods the template search by different BLAST runs and free available software like SwissModel, Pcons, ModWeb and other tools are used. Nevertheless, the generated model is not the native conformation and has to be analyzed with other software until a stable conformation of the structure can be predicted. Depending on the time which is provided the generated model is a good approach for the aim this master thesis has. KW - Hitzeschock-Proteine KW - Sequenzanalyse KW - Ligand KW - Strukturmodell KW - Chemotherapie Y2 - 2012 U6 - https://nbn-resolving.org/urn:nbn:de:bsz:mit1-opus-24536 UN - https://nbn-resolving.org/urn:nbn:de:bsz:mit1-opus-24536 ER -