TY  - THES
U1  - Bachelor Thesis
A1  - Kreszies, Tino
T1  - Experimental validation of RNA interference efficiency and off-target prediction in barley
N2  - This Bachelor thesis provides an experimental validation of the “si-Fi” software, which was designed for RNAi off-target searches and silencing efficiency predictions. The experimental approach is based on using synthetic DNA as RNAi-target as well as RNAi-trigger sequence. The data was generated by two different types of experiments using a transient gene silencing system in bombarded barley epidermal cells. The efficiency of RNAi was estimated by scoring the effect of silencing of the susceptibility-related gene Mlo on resistance of transformed cells to the powdery mildew fungus Blumeria graminis f. sp. hordei by observing reduction of fluorescent signals coming from an RNAi target fused to the green fluorescent protein. The aim of this work was a comparison between in silicio prediction of RNAi efficiency and off-target effects in barley and experimental data.
KW  - RNS-Interferenz
Y2  - 2013
U6  - https://nbn-resolving.org/urn:nbn:de:bsz:mit1-opus-41215
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:mit1-opus-41215
ER  -