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Optimization of an optical setup for combined TIRF/confocal fluorescence microscopy
- This work deals with the construction of a microscope for combined total internal reflection fluorescence (TIRF) and confocal microscopy. It is especially designed for single-molecule fluorescence spectroscopy. The design of the microscope body is based on the miCube (Hohlbein lab, Wageningen University, NL). The excitation and detection pathways were adapted to allow both TIRF and confocal illumination as well as camera and pointdetection for two color-channels to allow single-molecule Förster resonance transfer measurements
Author: | Hannes Michaelsen, Richard Börner |
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DOI: | https://doi.org/10.48446/opus-12286 |
ISSN: | 1437-7624 |
Parent Title (German): | 26. Interdisziplinäre Wissenschaftliche Konferenz Mittweida |
Publisher: | Hochschule Mittweida |
Place of publication: | Mittweida |
Document Type: | Conference Proceeding |
Language: | English |
Year of Completion: | 2021 |
Publishing Institution: | Hochschule Mittweida |
Contributing Corporation: | Laserinstitut Hochschule Mittweida |
Release Date: | 2021/05/17 |
Tag: | TIRFM |
GND Keyword: | Mikroskopie; Konfokale Mikroskopie |
Issue: | 002 |
Page Number: | 3 |
First Page: | 234 |
Last Page: | 236 |
Open Access: | Frei zugänglich |
Licence (German): | Urheberrechtlich geschützt |