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Zellfreie Proteinsynthese : Etablierung eines Werkzeugs der synthetischen Biologie

Cell-free protein synthesis : establishment of a synthetic biology tool

  • Cell-free protein synthesis is gaining increasing importance in basic and applied research as a result of advances that have been made in the past years. This study successfully established a commercially available coupled in vitro transcription/translation system for DNA expression templates based on HeLa cell lysate and suitable detection methods. Possibilities to establish an inexpensive in-house CFPS system based on E. coli lysate were elucidated. Cell-free extract from E. coli strains HB101, JM109 and B (Luria) was generated using sonication and a streamlined procedure for the creation of a reaction mix for convenient and simple application in CFPS systems was established. Of the three strains tested using the in-house protocol, neither exhibited any CFPS activity.

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Author:Robert Leidenfrost
Document Type:Master's Thesis
Year of Completion:2015
Granting Institution:Hochschule Mittweida
Release Date:2016/07/07
GND Keyword:Proteindesign; Proteine; Proteinsynthese
Institutes:03 Mathematik / Naturwissenschaften / Informatik
Dewey Decimal Classification:572.8 Molekularbiologie, Epigenetik
Open Access:Innerhalb der Hochschule
Licence (German):License LogoEs gilt das UrhG