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Characterization of the zebrafish larval mutant knörf

  • The larval zebrafish mutant Knörf has got a not yet identified gen, which is lethal after 14 dpf in a homozygous state. The mutation courses various degenerations and the loss of the regeneration ability. One of these degenerations was first discovered in the retina by a histological section. The mutants retinas show gaps in the IPL at 7 and 8 dpf which number increases during the maturation of the larva. In recent studies a pax 6 staining was performed, which showed that amacrine cells areaffected. Different types of amacrine cells were tested and it was shown that the parvalbuminergic amacrine cells disappear. The staining was performed in a time course. At 5 dpf is no difference between the number of parvalbuminergic amacrine cells in siblings and mutants but then the degeneration starts. At 2 dpa there is thefirst significant difference which increases at later stages and leads nearly to a full disappearance of these cells in the eye. Parvalbumin is not only present in the retina, therefore the brain as another central nervous system structure was examined. In the telencephalon these cells disappear already at 2 dpa. The parvalbuminergic cells are also present in the skeletal muscle of the tail. Here the degeneration starts approximately at the half of the tail and intensifies to distal areas. It was shown, that parvalbuminergic cells in the muscle disappear until 4dpa. The role of parvalbumin is seemed in the binding ofcalcium and therefore it supports the adjustment of the resting potential after an excitation in the central nervous system. In muscles it assists in the slowing of relaxing after a contraction of a muscle.

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Author:Mandy Schönbrodt-Rühl
Document Type:Bachelor Thesis
Date of Publication (online):2013/02/21
Publishing Institution:Hochschule Mittweida
Release Date:2013/02/21
GND Keyword:Zebrabärbling; Mutante; Parvalbumine; Degeneration
Institutes:03 Mathematik / Naturwissenschaften / Informatik
DDC classes:570 Biowissenschaften, Biologie
Open Access:Frei zugänglich
Licence (German):License LogoUrheberrechtlich geschützt