OPUS


Experimental validation of RNA interference efficiency and off-target prediction in barley

  • This Bachelor thesis provides an experimental validation of the “si-Fi” software, which was designed for RNAi off-target searches and silencing efficiency predictions. The experimental approach is based on using synthetic DNA as RNAi-target as well as RNAi-trigger sequence. The data was generated by two different types of experiments using a transient gene silencing system in bombarded barley epidermal cells. The efficiency of RNAi was estimated by scoring the effect of silencing of the susceptibility-related gene Mlo on resistance of transformed cells to the powdery mildew fungus Blumeria graminis f. sp. hordei by observing reduction of fluorescent signals coming from an RNAi target fused to the green fluorescent protein. The aim of this work was a comparison between in silicio prediction of RNAi efficiency and off-target effects in barley and experimental data.

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Metadaten
Author:Tino Kreszies
URN:urn:nbn:de:bsz:mit1-opus-41215
Document Type:Bachelor Thesis
Language:English
Year of Completion:2013
Publishing Institution:Hochschule Mittweida
Release Date:2014/06/04
GND Keyword:RNS-Interferenz
Institutes:03 Mathematik / Naturwissenschaften / Informatik
Access Rights:Frei zugänglich
Licence (German):License LogoEs gilt das UrhG

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