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Generation of transgenic zebrafish lines using CRISPR/Cas9 technology

  • In this work, a transgenic zebrafish line that expresses the fluorophore dsRed under the endogenous zebrafish cochlin promotor is supposed to be established, using the CRISPR/Cas9 system. dsRed was cloned into a pBluescript vector, followed by the cloning of the cochlin locus into this vector. This bait construct was then supposed to be micro injected into wild type AB zebrafish embryos. The micro injection of Cas9 mRNA, single guide RNA and a bait construct was practiced with the tyrosinase gene, which was disrupted using CRISPR/Cas9.

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Author:Rachel Woidtke
Advisor:Röbbe Wünschiers, Stefan Hans
Document Type:Bachelor Thesis
Year of Completion:2021
Granting Institution:Hochschule Mittweida
Release Date:2023/06/22
GND Keyword:CRISPR/Cas-Methode
Institutes:Angewandte Computer‐ und Bio­wissen­schaften
DDC classes:572.8028 CRISPR/Cas-Methode
Open Access:Innerhalb der Hochschule
Licence (German):License LogoUrheberrechtlich geschützt