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This work deals with the construction of a microscope for combined total internal reflection fluorescence (TIRF) and confocal microscopy. It is especially designed for single-molecule fluorescence spectroscopy. The design of the microscope body is based on the miCube (Hohlbein lab, Wageningen University, NL). The excitation and detection pathways were adapted to allow both TIRF and confocal illumination as well as camera and pointdetection for two color-channels to allow single-molecule Förster resonance transfer measurements
Decentralization is one of the key attributes associated with blockchain technology. Among the different developments in recent years, decentralized autonomous organizations (DAOs) have been of growing interest. DAOs are currently a key part of another emerging use case, namely decentralized science (DeSci). Given the novelty of the field, an integrative definition of DeSci has not been established, but some inherent concepts and ideas can be traced back to the Open Science movement. Although the DeSci movement has the potential to benefit the public, for example through funding underrepresented research areas or more inclusive and transparent research in general, some negative aspects of decentralization should not be neglected. Due to the novelty of blockchain and emerging use cases, research can and should precede mass adoption, to which this paper aims to contribute.
Mathematics behind the Zcash
(2020)
Among all the new developed cryptocurrencies, Zcash comes out to be the strongest cryptocurrency providing both transparency and anonymity to the transactions and its users by deploying the strong mathematics of zk-SNARKs. We discussed the zero knowledge proofs as a building block for providing the functionality to zk-SNARKs. It offers schnorr protocol which is further used in Zcash transactions where the validation of sent transaction is proved by cryptographic proof. Further, we deploy zk-SNARKs following common reference string that allows sender to prove that she knows a secret such that the proof is succinct, can be verified and does not leak the secret. Non-malleability, small proofs and effective verification make zk-SNARKs a classic tool in Zcash. We deal with NP problems therefore we have considered the elliptic curve cryptography to provide the security. Lastly, we explain Zcash transaction, the corresponding transaction completely hides the sender, receiver and amount of transaction using zero knowledge proof.
Reducing costs is an important part in todays business. Therefore manufacturers try to reduce unnecessary work processes and storage costs. Machine maintenance is a big, complex, regular process. In addition, the spare parts required for this must be kept in stock until a machine fails. In order to avoid a production breakdown in the event of an unexpected failure, more and more manufacturers rely on predictive maintenance for their machines. This enables more precise planning of necessary maintenance and repair work, as well as a precise ordering of the spare parts required for this. A large amount of past as well as current information is required to create such a predictive forecast about machines. With the classification of motors based on vibration, this paper deals with the implementation of predictive maintenance for thermal systems. There is an overview of suitable sensors and data processing methods, as well as various classification algorithms. In the end, the best sensor-algorithm combinations are shown.
We demonstrate a thulium-based fiber amplifier delivering pulses tunable between <120fs and 2ps duration at up to 228 μJ of pulse energy at a center wavelength of 1940 nm and 500-kHz repetition rate. Due to the excellent long-term stability, this system proves the ability of this technology to be integrated into ultra-fast material processing machines.
Pulsed laser processing of vacuum component surfaces is a promising method for electron cloud mitigation in particle accelerators. By generating a hierarchically structured surface, the escape probability of secondary electrons is reduced. The choice of laser treatment parameters – such as laser power, scanning speed and line distance – has an influence on the resulting surface morphology as well as on its performance. The impact of processing parameters on the surface properties of copper is investigated by Secondary Electron Yield (SEY) measurements, Scanning Electron Microscopy (SEM), ablation depth measurements in an optical microscope and particle release analysis. Independent of the laser wavelength (532nm and 1064nm), it was found that the surface morphology changes when varying the processing parameters. The ablation depth increases and the SEY reduces with increasing laser fluence. The final application requires the capability to treat tens of meters of vacuum pipes. The limiting factors of this type of surface treatment for the applicability in particle accelerators are discussed.
While blockchain technology is still in an early stage of its development, it is already of surging economic importance.
In the literature, blockchain is referred to as either being a disruptive, institutional, foundational, or general purpose technology. There is still no consensus about the economic theory that should apply for analyzing its economic effects. This article draws on use cases from the coffee supply chain to explore, which theories could potentially apply to an emerging blockchain economy.
Marker-based systems can digitally record human movements in detail. Using the digital biomechanical human model Dynamicus, which was developed by the Institut für Mechatronik, it is possible to model joint angles and their velocities such accurately that it can be used to improve motion analysis in competitive sports or for ergonomic evaluation of motion sequences. In this paper, we use interpretable machine learning techniques to analyze the gait. Here, the focus is on the classification between foot touchdown and drop-off during normal walking. The motion data for training the model is labeled using force plates. We analyze how we could apply our machine learning models directly on new motion data recorded in a different scenario compared to the initial training, more precise on a treadmill. We use the properties of the interpretable model
to detect drift and to transfer our model if necessary.
For monitoring laser beam welding processes and detecting or actively avoiding process defects, acoustic based measurements can be used in addition to optical measurement methods such as pyrometry. To reliably detect process events, it is essential to position the respective sensors in such a way that specific signal characteristics are reproducible and significant. However, there are only few investigations regarding the positioning for airborne sound sensors, especially for the detection of process emissions in the ultrasonic range. Therefore, in this research, the influence of the process distance as well as the angle and orientation of the microphone to a laser beam deep penetration welding process is investigated with respect to the detectability of process emissions in different frequency bands. It is shown that for a wide ultrasonic range a flat sensor angle with respect to the sample surface leads to an increased signal strength of the acoustic emissions compared to steep angles.
In this paper, we designed, implemented, and tested a special surveillance camera system based on a combination of classical image processing algorithms. The system’s sub-objective consists of tracking experimental vehicles driving on a defined trajectories (Rail) in real time. Furthermore, it analyzes the scene to collect additional vehicles & rail-related information. The system then uses the gathered data to reach its main objective which confines oneself in independently predicting vehicles collision. Consequently, we propose a hybrid method of detecting and tracking ATLAS-vehicles efficiently. To detect the vehicle at the beginning of the video, periodically every n-frame, and in the case where the tracked vehicle has been lost, we used Histogram Back-Projection. By contrast, Kernelized correlation filter is used to track the detected vehicles. Combining these two methods provides one of the best trade-offs between accuracy and speed even on a single processing core. The proposed method achieves the best performance compared with three different approaches on a custom dataset.