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Footage of organoids taken by means of fluorescence microscopy and segmented as well as triangulated by image analysis software like LimeSeg and Mastodon often needs to be visualized in aesthetic manner for presentation of the results in scientific papers, talks and demonstrations. The goal of this work was to create a simple to use addon “Biobox” for the open source 3D – visualization package “Blender” which would allow to import triangulated 3D data with animation over time (4D), produced by image analysis software, and optimize it for efficient usage. ”Biobox” offers several visualization tools for the creation of rendered images and animation videos by biologists.
The optimization of imported data was performed by using Blender intern modifiers. The optimized data can then be visualized by using several tools built for visualizing the organoid in frozen, animated and semi-transparent manners. A dynamic link for object selection and dynamic data exchange between Blender and Mastodon was developed. Additionally, a user interface was developed for manual correction errors of segmentation and steering the object detection algorithms of LimeSeg. The benchmark of the developed addon “Biobox” was performed on real scientific data. The benchmark test demonstrated that developed optimization result in significant (~5 fold) decrease of RAM usage and acceleration of visualization more than 160 times.
The number of Internet of Things (IoT) devices is increasing rapidly. The Trustless Incentivized Remote Node Network, in short IN3 (Incubed), enables trustworthy and fast access to a blockchain for a large number of low-performance IoT devices. Although currently IN3 only supports the verification of Ethereum data, it is not limited to one blockchain due to modularity. This thesis describes the fundamentals, the concept and the implementation of the Bitcoin verification in IN3.
In this thesis two novel methods for removing undesired background illumination are de-veloped. These include a wavelet analysis based approach and an enhancement of a deep learning method. These methods have been compared with conventional methods, using real confocal microscopy images and synthetic generated microscopy images. These synthetic images were created utilizing a generator introduced in this thesis.
The Tutte polynomial is an important tool in graph theory. This paper provides an introduction to the two-variable polynomial using the spanning subgraph and rank-generating polynomials. The equivalency of definitions is shown in detail, as well as evaluations and derivatives. The properties and examples of the polynomial, i.e. the universality, coefficient relations, closed forms and recurrence relations are mentioned. Moreover, the thesis contains the connection between the dichromate and other significant polynomials.
The GeoFlow II experiment aims to replicate Earth’s core dynamics using a rotating spherical container with controlled temperature differences and simulated gravity. During the GeoFlow II campaign, a massive dataset of images was collected, necessitating an automated system for image processing and fluid flow visualization in the northern hemisphere of the spherical container. From here, we aim to detect the special structures appearing on the post processed images. Recognizing YOLOv5’s proficiency in object detection, we apply Yolov5 model for this task.
In the field of satellites it is common practice to combine multiple ground stations into one network, to increase communication times with satellites. This work focuses on TIM, which is an international academic colaborative project. Important criteria for this project are elaborated and used to evaluate existing ground station networks. It concludes that there is no appropriate solution availiable for this specific use case and establish a proposed solution. The proposed ground station network software will be elaborated and evaluated.
The games industry has significantly grown over the last 30 years. Projects are getting bigger and more expensive, making it essential to plan, structure and track them more efficiently.
The growth of projects has increased the administrative workload for producers, project managers and leads, as they have to monitor and control the progress of the project in order to keep a permanent overview of the project. This is often accompanied by a lack of insight into the project and basic communication within the team. Therefore, the goal of this thesis is to enhance conventional project management methods with process structures that occur frequently in game development.
This thesis initially elaborates on what project management in the game industry actually is: Here, methods are considered, especially agile methods and progress tracking prac-tices, which were created for software development and have become a standard in game development. Subsequently, an example is used to demonstrate how process management can function within the development of video games. Based on this, the ideal is depicted, which is implemented and used in a tool at the German games studio KING Art GmbH. This ideal is compared with expert interviews in order to verify its gen-eral validity in the industry.
By integrating process structures, the administrative effort can be reduced, communica-tion within game development can be simplified, while the current project status can be permanently presented. This benefits both project management and leads, as well as the entire team. Further application tests of this theory would have to be organized to check scalability and to draw comparisons to other applications.
Since its foundation as an application of algebra, coding theory is obtaining a day by day increasing importance. For instance, any communication system needs the concepts of coding theory to function efficiently. In this thesis, reader will find an introductory explanation to linear codes and binary hamming codes including some of the algebraic tools devised in their applications. All the described software applications are verified using SageMath 9.0 using Hochschule Mittweida’s JupyterHub.
In this work, a transgenic zebrafish line that expresses the fluorophore dsRed under the endogenous zebrafish cochlin promotor is supposed to be established, using the CRISPR/Cas9 system. dsRed was cloned into a pBluescript vector, followed by the cloning of the cochlin locus into this vector. This bait construct was then supposed to be micro injected into wild type AB zebrafish embryos. The micro injection of Cas9 mRNA, single guide RNA and a bait construct was practiced with the tyrosinase gene, which was disrupted using CRISPR/Cas9.
The objective of this Bachelor Project is the creation of a tool that should support forensic investigators during IT forensic interventions. It uses Kismet as the base program and adds functionalities to it via the plugin interface. The installation of the plugin shall be explained, how the plugin works, and a recommendation on how to use it. To understand the underlying basics, an introduction about WLAN and Bluetooth is given. The tests that were performed with the new plugin are described as well as their results. It is therefore briefly discussed why the tool is applicable for locating Wi-Fi devices, especially access points, but not Bluetooth devices. Using all this a few ideas on how to improve the tool and what can be researched in this area are provided.