Refine
Document Type
- Bachelor Thesis (2) (remove)
Year of publication
- 2013 (2) (remove)
Language
- English (2) (remove)
Keywords
- Embryonalentwicklung (1)
- Gerste (1)
- In silico-Methode (1)
- Mikrospore (1)
- Pollen (1)
- RNS-Interferenz (1)
Institute
- 03 Mathematik / Naturwissenschaften / Informatik (2) (remove)
The main purpose of this Bachelor thesis was to find and to compile comprehensive information on barley genes expressed in the context of pollen embryogen esis. In the present study, this approach was confined to genes that were previously known to be associated with the initiation of embryogenesis in different plant species. First, candidate transcript sequences were identified in barley. Second, transcript and associated genomic sequences were analyzed in silico to provide suitable structural and functional annotations. Finally, the results of one representative example are presented and interpreted in detail. This work aims to contribute to a significantly improved understanding of pollen embryogenesis - a biological phenomenon broadly used for haploid technology in crop improvement.
This Bachelor thesis provides an experimental validation of the “si-Fi” software, which was designed for RNAi off-target searches and silencing efficiency predictions. The experimental approach is based on using synthetic DNA as RNAi-target as well as RNAi-trigger sequence. The data was generated by two different types of experiments using a transient gene silencing system in bombarded barley epidermal cells. The efficiency of RNAi was estimated by scoring the effect of silencing of the susceptibility-related gene Mlo on resistance of transformed cells to the powdery mildew fungus Blumeria graminis f. sp. hordei by observing reduction of fluorescent signals coming from an RNAi target fused to the green fluorescent protein. The aim of this work was a comparison between in silicio prediction of RNAi efficiency and off-target effects in barley and experimental data.