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There are a lot of people taking part in more than one competition. The competitions are also of a different kind. From local events with a small number of participants to international tournaments watched by many viewers. Naturally it becomes necessary a system to assess and compare the success in various competitions.
The existing ranking systems are usually specialized to fit their application area. More general ranking methods also exist. They can be applied to a wide spectrum of competition fields. However these ranking methods are still not universal and don't cover some important features of the competitions.
A totally new ranking system has been developed within the present master thesis. Its primary purpose is to evaluate and measure prestige gained by participants in competitions. The main contribution of the thesis consists of an original mathematical model that makes the ranking system unique.
The developed ranking system claims to be universal and interdisciplinary. It is based on the fundamental element that distinguishes the competition from the non-competition areas, namely standings that rank the participants according to their performance. The universality and the interdisciplinarity of the ranking system make available cross-disciplinary comparisons, which is usually very subjective and difficult for implementation.
The contribution of the master thesis extends beyond the theoretical area. A ranking software that fully implements this novel ranking system has been designed and developed. The software makes the practical benefits of the ranking system immediately available to potential application areas such as sports clubs and universities.
And finally, the developed ranking system offers a new viewpoint to the competitions – as a way of gaining prestige, rather than the traditional viewpoint of demonstrating mastery.
The main purpose of this Bachelor thesis was to find and to compile comprehensive information on barley genes expressed in the context of pollen embryogen esis. In the present study, this approach was confined to genes that were previously known to be associated with the initiation of embryogenesis in different plant species. First, candidate transcript sequences were identified in barley. Second, transcript and associated genomic sequences were analyzed in silico to provide suitable structural and functional annotations. Finally, the results of one representative example are presented and interpreted in detail. This work aims to contribute to a significantly improved understanding of pollen embryogenesis - a biological phenomenon broadly used for haploid technology in crop improvement.
This Bachelor thesis provides an experimental validation of the “si-Fi” software, which was designed for RNAi off-target searches and silencing efficiency predictions. The experimental approach is based on using synthetic DNA as RNAi-target as well as RNAi-trigger sequence. The data was generated by two different types of experiments using a transient gene silencing system in bombarded barley epidermal cells. The efficiency of RNAi was estimated by scoring the effect of silencing of the susceptibility-related gene Mlo on resistance of transformed cells to the powdery mildew fungus Blumeria graminis f. sp. hordei by observing reduction of fluorescent signals coming from an RNAi target fused to the green fluorescent protein. The aim of this work was a comparison between in silicio prediction of RNAi efficiency and off-target effects in barley and experimental data.