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Optimization of an optical setup for combined TIRF/confocal fluorescence microscopy

  • This work deals with the construction of a microscope for combined total internal reflection fluorescence (TIRF) and confocal microscopy. It is especially designed for single-molecule fluorescence spectroscopy. The design of the microscope body is based on the miCube (Hohlbein lab, Wageningen University, NL). The excitation and detection pathways were adapted to allow both TIRF and confocal illumination as well as camera and pointdetection for two color-channels to allow single-molecule Förster resonance transfer measurements

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Author:Hannes Michaelsen
Parent Title (German):26. Interdisziplinäre Wissenschaftliche Konferenz Mittweida
Publisher:Hochschule Mittweida
Place of publication:Mittweida
Document Type:Conference Proceeding
Year of Completion:2021
Publishing Institution:Hochschule Mittweida
Contributing Corporation:Laserinstitut Hochschule Mittweida
Release Date:2021/05/17
GND Keyword:Mikroskopie; Konfokale Mikroskopie
Page Number:3
First Page:234
Last Page:236
Open Access:Frei zugänglich
Licence (German):License LogoEs gilt das UrhG