Zellfreie Proteinsynthese : Etablierung eines Werkzeugs der synthetischen Biologie
Cell-free protein synthesis : establishment of a synthetic biology tool
- Cell-free protein synthesis is gaining increasing importance in basic and applied research as a result of advances that have been made in the past years. This study successfully established a commercially available coupled in vitro transcription/translation system for DNA expression templates based on HeLa cell lysate and suitable detection methods. Possibilities to establish an inexpensive in-house CFPS system based on E. coli lysate were elucidated. Cell-free extract from E. coli strains HB101, JM109 and B (Luria) was generated using sonication and a streamlined procedure for the creation of a reaction mix for convenient and simple application in CFPS systems was established. Of the three strains tested using the in-house protocol, neither exhibited any CFPS activity.
Author: | Robert Leidenfrost |
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Document Type: | Master's Thesis |
Language: | German |
Year of Completion: | 2015 |
Granting Institution: | Hochschule Mittweida |
Release Date: | 2016/07/07 |
GND Keyword: | Proteindesign; Proteine; Proteinsynthese |
Institutes: | 03 Mathematik / Naturwissenschaften / Informatik |
DDC classes: | 572.8 Molekularbiologie, Epigenetik |
Open Access: | Innerhalb der Hochschule |
Licence (German): | Urheberrechtlich geschützt |