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Our current research aims to establish a complete ribonucleic acid (RNA) production line from plasmid design to purification of in vitro transcribed RNA and labeling of RNA. RNA is the central molecule within the central dogma of molecular biology and is involved in most essential processes within a cell[1]. In many cases, only compact three-dimensional structures of the respective RNA are able to fulfill their function. In this context, RNA tertiary contacts such as kissing loops and pseudoknots are essential to stabilize three-dimensional folding[2]. We will produce a tertiary contact consisting of a kissing loop and a GAAA tetraloop that occurs in eukaryotic ribosomal RNA[3,4]. The RNA sequence is integrated into a vector plasmid. Subsequently, the plasmid is amplified in E. coli. After following plasmid purification steps, the RNA sequence will be transcribed in vitro[5,6]. In order for the RNA be used for Förster resonance energy transfer (FRET) experiments at the single molecule level, fluorescent dyes must be coupled to the RNA molecule[7].