Generation of transgenic zebrafish lines using CRISPR/Cas9 technology
- In this work, a transgenic zebrafish line that expresses the fluorophore dsRed under the endogenous zebrafish cochlin promotor is supposed to be established, using the CRISPR/Cas9 system. dsRed was cloned into a pBluescript vector, followed by the cloning of the cochlin locus into this vector. This bait construct was then supposed to be micro injected into wild type AB zebrafish embryos. The micro injection of Cas9 mRNA, single guide RNA and a bait construct was practiced with the tyrosinase gene, which was disrupted using CRISPR/Cas9.
Author: | Rachel Woidtke |
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Advisor: | Röbbe Wünschiers, Stefan Hans |
Document Type: | Bachelor Thesis |
Language: | English |
Year of Completion: | 2021 |
Granting Institution: | Hochschule Mittweida |
Release Date: | 2023/06/22 |
GND Keyword: | CRISPR/Cas-Methode |
Institutes: | Angewandte Computer‐ und Biowissenschaften |
DDC classes: | 572.8028 CRISPR/Cas-Methode |
Open Access: | Innerhalb der Hochschule |
Licence (German): | Urheberrechtlich geschützt |